Docking sites inside Cas9 for adenine base editing diversification and RNA off-target elimination
نویسندگان
چکیده
منابع مشابه
Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases
SUMMARY The Type II clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system is an adaptive immune response in prokaryotes, protecting host cells against invading phages or plasmids by cleaving these foreign DNA species in a targeted manner. CRISPR/Cas-derived RNA-guided engineered nucleases (RGENs) enable genome editing in cultured cells, animals and plants, but are limite...
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Adenosine (A) to inosine (I) RNA editing occurs widely in the human transcriptome, and a large proportion of editing sites are within untranslated regions (UTRs). MicroRNAs (miRNAs), an abundant class of regulatory genes, specify the expression of a large number of target genes by pairing to their 3' UTRs. To study the interplay between these two post-transcriptional events, we developed a comp...
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The CRISPR/Cas9 system is a bacteria immune system protecting cells from foreign genetic elements and has been modified to edit genomes in targeted locations. However, the risk of binding at off-target locations limits its power and thus it is necessary to identify these off-targets. Finding offtargets is computationally expensive, especially when allowing several mismatches. We present the CRI...
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Gene modifications in animal models have been greatly facilitated through the application of targeted genome editing tools. The prokaryotic CRISPR/Cas9 type II genome editing system has recently been applied in cell lines and vertebrates. However, we still have very limited information about the efficiency of mutagenesis, germline transmission rates and off-target effects in genomes of model or...
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CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated Cas9) has been widely used in genome editing in a variety of organisms, including rice (Cong et al., 2013; Feng et al., 2013). The majority of the editing events reported in plants typically result in an abundance of random insertions and deletions (Indels) at the target locus through the error-prone non-ho...
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ژورنال
عنوان ژورنال: Nature Communications
سال: 2020
ISSN: 2041-1723
DOI: 10.1038/s41467-020-19730-9